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Literature summary extracted from

  • Silvaroli, J.A.; Pleshinger, M.J.; Banerjee, S.; Kiser, P.D.; Golczak, M.
    Enzyme that makes you cry-crystal structure of lachrymatory factor synthase from Allium cepa (2017), ACS Chem. Biol., 12, 2296-2304 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.12 expressed in Escherichia coli BL21(DE3) cells Allium cepa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.99.12 apo-form and in complex with crotyl alcohol, sitting drop vapor diffusion method, using 0.1 M sodium acetate at pH 4.5 containing PEG 3350 at concentrations between 25% and 32% (w/v) Allium cepa

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.12 R71L/E88Q the mutations completely abolish the enzymatic reaction Allium cepa

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.99.12 crotyl alcohol i.e. (2E)-but-2-en-1-ol Allium cepa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.12 (E)-prop-1-en-1-SO-peroxol Allium cepa
-
 (Z)-propanethial S-oxide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.12 Allium cepa P59082
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.12 glutathione-Sepharose resin column chromatography, Superdex 200 gel filtration, and HiTrap Q ion exchange column chromatography Allium cepa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.12 (E)-prop-1-en-1-SO-peroxol
-
 Allium cepa (Z)-propanethial S-oxide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.99.12 lachrymatory factor synthase
-
 Allium cepa
5.3.99.12 LFS
-
 Allium cepa